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The term shRNA, as used herein, refers to an RNA agent having a stem loop structure, comprising a first and second region of complementary sequence, the degree of complementarity and orientation of the regions being sufficient such that base pairing occurs between the regions, the first and second regions being joined by a loop region, the loop resulting from a lack of base pairing between nucleotides or nucleotide analogs within the loop region <a href=http://buycialis.buzz>cialis vs viagra</a> Sample size calculation was based on the primary outcome, CLBR at 6 months from randomization, and using the following estimates from the literature